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Optimizing fermentation strategies for production of biosurfactants utilized in oil bioremediation


There are over 3,000 gas stations in Taiwan,containing more than 10,000 oil storage tanks underneath. About half of the gas stations have been operated for over ten years,and a recent investigation by Taiwan's EPA showed that leaking problems began to occur at a number of aged oil storage tanks,causing oil contamination in nearby soils and groundwater. Therefore,extremely critical to clean up and soundly control the oil-contaminated sites before the situation further deteriorates. Biourfactants could increase solubility of oils in water to enhance the bioavailability of the hydrophobic substrates,leading to higher oil degradation rates. Thus,biosurfactants,natural products of variety of microorganisms,appear preferable for the environmental applications. In addition to bioremediation,biosurfactants also find their applications in medical,cosmetic,and food industries,etc. However,the factors restricting commercial application of biosurfactants have been the low yield and high cost for producing biosurfactants. Therefore,there is an urgent need to develop an efficient and cost-effective bioprocess for the production of biosurfactants. This study demonstrated a powerful and feasible way to produce biosurfactant from Bacillus subtitles more efficiently through a solid-carrier-stimulating fermentation. Addition of 25 g/L of activated carbon into fermentation broth triggered a remarkable enhancement in biosurfctant production from 100 to 3600mg/L. Moreover,this study utilized a modified ferment or module to enhance biosurfactants production. The ferment or could solve the problem of sever foaming and enhanced cell concentration,thereby increasing biosurfactant production dramatically. Under the modified ferment or,a maximum overall productivity(106 mg/L/h) and biosurfactant concentration(6450 mg/L) were achieved. These results demonstrate the potential of low-cost and higher-rate production of biosurfactant.......

【作者名称】: Wen-Fu Xin, Yu-Hong Wei
【作者单位】: Grauduate School of Biotechnology and Bioengineering,Yuan Ze University,Chung-Li 32003,Taiwan, Grauduate School of Biotechnology and Bioengineering,Yuan Ze University,Chung-Li 32003,Taiwan
【关 键 词】: 生物表面活性剂, 石油污染, 生物修复, 土壤污染
【会议名称】: 第五届全国化工年会论文集
【期刊论文数据库】: [DBS_Articles_01]
【期刊论文编号】: 100,000,009
【摘要长度】: 1,935
【会议地点】: 西安
【会议组织】: 中国化工学会
【会议时间】: 2008
【上篇论文】: 中文会议 - Thermal and mechanical properties of the plapeg blend and the plapcl blend both prepared by twin screw extrusion
【下篇论文】: 中文会议 - Synthesis and Antitumor Activity of Novel Spin Labeled Podophyllotoxin Derivatives

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