Impact of Different Methods of Soil Sampling and DNA Extraction on the Identification of Soil Bacterial Abundance under Elevated CO2
Elevated atmospheric CO, (eCO,)is anticipated to have marked effects on soil microbial populations. Whilethere is experimental evidence to support this view there are also studieswhere changes in microbial populations, such as the abundance of bacteria, aresuggested but cannot be statistically established. We conducted this study toidentify whether the sampling and sample treatment methods used could influencethe results obtained using bacterial abundance as the variable of interest. Wetested three different sampling methods and two different DNA extraction kits.The first because microbes are distributed heterogeneously in soil so thesampling procedure might be expected to influence the accuracy and precision ofthe population estimate and the second because the quantity and quality of DNAextracted influences the microbial analyses that can be performed and can introducebias. Samples were taken from a long-running FACE experiment on grassland fromunder plants of ,.We found tha,t bacteri......
【作者名称】: Fengxia Li
, Saman Bowatte
, Paul C. D. Newton
, Dongwen Luo
【关 键 词】: DNA Extraction Kit
, DNA Yield
, DNA Quality
, Gene Abundance
, Bacterial 16S rRNA
, Free Air Carbon Dioxide Enrichment
【上篇论文】: 外军国防科技报告 - Barriers to the Implementation of the Nursing Approach in Public Hospitals in Lubumbashi in the Democratic Republic of Congo: A Cross-Sectional Descriptive Study
【下篇论文】: 外军国防科技报告 - Study to Define Frequency in Routine Analytical Controls in the Radiolabelling Process
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