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Impact of Different Methods of Soil Sampling and DNA Extraction on the Identification of Soil Bacterial Abundance under Elevated CO2


Elevated atmospheric CO, (eCO,)is anticipated to have marked effects on soil microbial populations. Whilethere is experimental evidence to support this view there are also studieswhere changes in microbial populations, such as the abundance of bacteria, aresuggested but cannot be statistically established. We conducted this study toidentify whether the sampling and sample treatment methods used could influencethe results obtained using bacterial abundance as the variable of interest. Wetested three different sampling methods and two different DNA extraction kits.The first because microbes are distributed heterogeneously in soil so thesampling procedure might be expected to influence the accuracy and precision ofthe population estimate and the second because the quantity and quality of DNAextracted influences the microbial analyses that can be performed and can introducebias. Samples were taken from a long-running FACE experiment on grassland fromunder plants of ,.We found tha,t bacteri......

【作者名称】: Fengxia Li, Saman Bowatte, Paul C. D. Newton, Dongwen Luo
【关 键 词】: DNA Extraction Kit, DNA Yield, DNA Quality, Gene Abundance, Bacterial 16S rRNA, Free Air Carbon Dioxide Enrichment
【网站名称】: OALIB数据库
【期刊论文数据库】: [DBS_Articles_01]
【期刊论文编号】: 103,633,806
【摘要长度】: 1,000
【栏目名称】: 所有文件
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