Anti-Tumor Effect and Mechanism of Parthenolide in Gastric Cancer Cellline BGC-823
Objective: To study the anti-tumor effect and mechanism of parthenolide in gastric cancer cell BGC-823. Methods: The cck8 assay was used to detect the changes of BGC-823 cells viability after treatment with different con-centrations of parthenolide at different time points. The proliferation ability of BGC-823 cells was detected by clone formation assay. And the cell cycle and apoptosis were measured by flow cy,tometry. Meanwhile, to detect the different in intracellular ROS production levels, the fluorescence assay was used. And with the help of western blotting, cell cycle- and apoptosis-related protein expression can be detected. Results: Parthenolide could inhibit the viability of BGC-823 cells in a dose- and time-dependent manner (P < 0.01). In BGC-823 cells exposed to parthenolide, the apoptosis rate was found significantly increased (P < 0.01), and the protein expression of cleaved-caspase3, cleaved-caspase8, and cleaved-caspase9 significantly increased (P < 0.01); the cell cycl......
【作者名称】: Wenhao Zhu
, Hanni Zhang
, Yunliang Guo
, Keli Ge
, Yonghong Zhou
【关 键 词】: Parthenolide
, Gastric Cancer
, Cell Proliferation
, Cell Cycle
, Open Access Library
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