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Cloning and Expression Analysis of TTG1 Gene Related to Rosa rugosa Trichomes Formation


The TTG1 transcription factor plays an important role in the formation of plant trichomes. Based on the R. rugosa transcriptome data, this study cloned a R. rugosa TTG1 gene, named RrTTG1, and carried out bioinformatics analysis and fluorescence quantitative analysis to explore the relationship between TTG1 gene and R. rugosa trichomes formation, in order to lay a good foundation to cultivate a thornless plant in the family Rosaceae. In this experiment, six hybrid cultivars of R. rugosa “Zizhi”, R. rugosa “Xizi”, R. rugosa “Tang fen”, R. rugosa “Hun chun”, R. rugosa “Zi long wo chi” and R. rugosa “Tian e huang” were used as experimental materials, and the cDNA full length of this gene was obtained by RT-PCR and RACE, and the full length of the cDNA was 1348 bp. After bioinformatics analysis, it is predicted that its molecular formula is C1723H2661N465O529S12, the molecular weight is 38.71 KB, and the isoelectric point is 5.00. Its instability index is 54.30, which belongs to unstable p......

【作者名称】: Yu Wang, Mingyuan Zhao, Zongda Xu, Lanyong Zhao, Xu Han
【关 键 词】: <, i>, R. rugosa<, /i>, Trichome, <
【网站名称】: 美国科学研究出版社
【期刊论文数据库】: [DBS_Articles_01]
【期刊论文编号】: 103,355,260
【摘要长度】: 1,000
【栏目名称】: 期刊
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